The Basic Principles Of flow cytometer
The Basic Principles Of flow cytometer
Blog Article
Knowledge-enriched characterization of cell populations. Morphological details from pictures can add on the richness of flow cytometry details. As an example, the determine demonstrates an or else standard apoptosis assay employing Annexin V and PI, adding cell imaging to characterize cells in Every single population to reveal morphologically unique characteristics. These insights couldn't have been received from multiplex staining alone.
No matter if you’re a novice or an expert, investigate our means to progress your research and knowledge in flow cytometry.
Blend acoustic focusing know-how with superior-parameter Examination for flexible spectral unmixing and conventional workflows.
Learn new insights into your experimental data by visualizing populations applying blended fluorescence and morphology details. Our computer software interface wizards enable downsampling, concatenation (merged information) and dimensionality equipment that are created into your program for an intuitive, streamlined information analysis working experience.
"An image isn't really very really worth one thousand dots, but it really genuinely helps you to see photos. It is great to find out illustrations or photos that correlate with staining. One example is, AnnexinV-beneficial cells which are morphologically unique than nutritious cells, or CD14+ monocytes which might be greater plus much more textured than CD3+/CD4+/CD14- lymphocytes.
Below, a qualified person has gated singlets confidently. After assessing the handbook singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate contains greater than four% aggregates.
Optimizing gating technique. Even strong manual singlet gating is mistake-inclined and stays a subjective choice stage in Pretty much all flow cytometry assays. Imaging can be used to verify and change gates to include only solitary cells of interest.
Our scientists will style panels to suit your needs as over and over you require—totally free, no solution invest in needed.
The Attune NxT cytometer is definitely the trustworthy workhorse instrument your lab can depend on for consistent and trustworthy efficiency. The Attune CytPix model features a superior-pace brightfield digicam and AI-driven graphic analysis computer software, with the latest upgrades including device learning resources and an intuitive person interface, enabling Increased facts precision, precision, and faster insights into sample biology.
Consumers can watch party illustrations or photos in true-time to substantiate sample quality, optimize protocols, and document the morphology of each and every cell populace discovered for Evaluation. Imaging capture also Added benefits from acoustic focusing, which really helps to situation the cells to ensure that a sharp, centered graphic is obtained.
AI-pushed automatic impression Examination Along with the Attune Cytometric Software program translates occasion functions into distinctive morphology parameters that can be coupled with regular fluorescence and scatter parameters to expose highly reproducible inhabitants variations and exceptional subsets.
We Earlier demonstrated the strength of imaging Motor vehicle-T/Ramos cell interactions. Allow’s look at just the populace of biggest fascination, the double constructive activities, to learn more. We are able to now use prolonged morphological parameters (in this article, circularity and skewness of intensity) to more study the options of such populations and refine gating on these situations, expanding facts robustness.
Built-in dimensionality reduction equipment elevated resolution into populations. click here Fluorescently gated populations again-gated on to UMAP plot to substantiate expected population resolution in lysed entire human blood. Consumer can watch activities from Just about every cluster for visual confirmation of mobile phenotypes.
No wash/no lyse leukocyte Investigation with imaging. Cells have been obtained from 24-hour-aged blood by dilution in 1 mM EDTA ( Specialist UX/UI allows people to teach AI designs for expanded applications. (A) The “Educate Design” feature provides annotation applications and serious-time model coaching precision.
Gating on these prolonged parameters enables you to quickly and properly discover populations of desire and make sure gating approach with little or no manual assessment. The Attune software package features entry to state-of-the-art machine Understanding Evaluation resources within an expertly created user interface, which makes it exceptionally convenient to use.
As the mobile line had previously been analyzed over the Attune CytPix Flow Cytometer, the scientists went again to the photographs and were being in the position to doc the microbial an infection at least five days previously.
Agilent Price Promise assures you at the least a decade of instrument use with the day you purchase. Or else, We're going to credit score you Along with the residual value of the program towards an upgraded product.
What seemed to be a homogeneous population is really extra varied—an Perception that needs to be regarded as when interpreting results.